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Pediatric Nephrology

Procedures - Instructions for Urine Testing

  • Fill the test tube 1/2 to 1/3 with urine.
  • Add without shaking 20 drops of sulfosalicylic acid (SSA).
  • Read the reaction as follows:
    Negative - No change in appearance of urine
    Trace - Very faint cloudiness
    1+ - Moderate cloudiness through which newspaper print can be read
    2+ - More marked cloudiness through which heavy black lines on a white paper are still visible
    3+ - White cloud through which black lines are not visible
    4+ - Fluffy white material which quickly sinks to the bottom of tube
    Please note: SSA acid is 3% solution for this.